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Home |Products|Coagulation Proteins|Human Protein C

Human Protein C

Cat.-Nr.: HCPC-0070

Description

The vitamin K-dependent zymogen, protein C, is synthesized in the liver as a single chain polypeptide and is subsequently converted to a disulfide linked heterodimer, by removal of a dipeptide (Lys-146 and Arg-147) from the precursor molecule (1,2). Trace quantities of the single chain form have been observed in plasma. The light chain, which is responsible for the calcium dependent binding of protein C to phospholipid vesicles, contains 11 -carboxyglutamic acid (gla) residues, 1 -hydroxyaspartic acid residue, and 2 epidermal growth factor (EGF) homology domains. The serine protease catalytic triad is located in the heavy chain. Human protein C is susceptible to proteolytic cleavage of a peptide (Mr = 3000) from the COOH-terminal end of the heavy chain, yielding an altered form referred to as -protein C. No functional distinction between - and -protein C has been observed. A single cleavage at Arg-12 (Arg-14 in bovine) of the heavy chain of human protein C converts the zymogen into the serine protease, activated protein C. This cleavage is catalyzed by a complex between -thrombin and the endothelial cell surface protein thrombomodulin. In contrast to the other vitamin K dependent coagulation factors, activated protein C functions as an anticoagulant by catalyzing the proteolytic inactivation of factors Va and VIIIa. APC also contributes to the fibrinolytic response by complex formation with plasminogen activator inhibitors. Bovine protein C is prepared from fresh citrated bovine plasma by a modification of the Walker procedure (3), as described by Haley et al. (4). Mouse protein C is prepared from fresh frozen citrated plasma using conventional chromatography techniques. Human protein C is prepared from fresh frozen citrated human plasma using a combination of immunoaffinity chromatography (5), and conventional techniques (4,9). Protein C is provided in 50% (vol/vol) glycerol/H2O and should be stored at -20°C. Purity is determined by SDS-PAGE analysis and activity is measured using a chromogenic substrate based assay.

  • SUPPLIER:

    Haematologic TechnologiesProlytix

  • STATUS:

    In Stock

  • SIZE:

    100 µg

  • Overview

Overview

  • Species: Human
  • Additional Attributes: Zymogenes
  • Formulation:50% (vol/vol) glycerol/H2O
  • Purity:>95%
  • Localization:Plasma
  • Concentration of plasma:4-5 µg/ml
  • Mode of action:Zymogen; precursor to the serine protease activated protein C (APC)
  • Molecular Weight:62000
  • Extinction Coeff:14,5
  • Isoelectric point:4.4-4.8
  • Structure:two chains, Mr=41,000 and 21,000, disulfide linked, NH2-terminal gla domain two EGF domains
  • Percent carbohydrate:0%
  • Post translationnal modifications:eleven gla residues (bovine), nine gla residues (human), one β-hydroxyaspartate

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Human Protein C, biotinylated Human Platelet Factor 4
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