Overview
- Species: Rat
- Keywords: 3D Hydrogels, Collagen, Extracellular Matrix
- Specific Attributes: Telocollagen/acidic treatment, Type I
- Product Type: Telocollagen, Type 1 / I
- Features: solution
- Packaging:Rat Tail Collagen Solution (10 mg/ml)
High Concentration Rat Tail Collagen 10 mg/mL
Cat.-Nr.: HCRTC-100MG
Description
RatCol® High Concentration Type I Acid Soluble Rat Tail Collagen (telocollagen) contains 100 mg at a concentration of approximately 10 mg/mL in a 0.02M acetic acid solution (~pH 3). RatCol® collagen is soluble telo-collagen. This collagen product is provided in user-friendly packaging for use and storage. This product is sterile filtered and is supplied as a ready to use solution.
This product is ideal for coating of surfaces, providing preparation of thin layers for culturing cells, or use as a solid gel. RatCol® collagen is suitable for applications using a variety of cell lines including hepatocytes, fibroblasts and epithelial cells.
| Parameter, Testing, and Method | RatCol® Type I Collagen #HCRTC-100 mg |
| Sterilization Method | Filtration |
| Extraction Method | Acid – telocollagen |
| Form | Solution |
| Package Size | 100 mg (~10 mL) Kit |
| Storage Temperature of Collagen | 2-10°C |
| Storage Temperature of Neutralization Solution | Room Temperature |
| Shelf Life | Minimum of 6 months from date of receipt |
| Collagen Concentration – Biuret | ~10.0 mg/mL |
| Collagen Purity – Silver Staining | >99% |
| pH | 3.0-3.8 |
| Kinetic Gel Test (Minutes) | <40 |
| Gel Formation Tube Test (Minutes) | <40 |
| Electrophoretic Pattern – Coomassie Blue | Characteristic |
| Sterility – USP modified | No growth |
| Endotoxin – LAL | <10.0 EU/mL |
| Osmolality (mOsmo H2O/kg) | <35 |
| Cell Attachment Assay | Pass |
| Source | Rat Tail Tendon |
| Hydrogel Young’s Modulus E (Pa) | Characteristic |
For an overview of Type I Collagen (Atelocollagen & Telocollagen) products (click)
SUPPLIER:
Advanced BioMatrix
STATUS:
In Stock
SIZE:
100 mg
RatCol® High Concentration Type I Acid Soluble Rat Tail Collagen (telocollagen) contains 100 mg at a concentration of approximately 10 mg/mL in a 0.02M acetic acid solution (~pH 3).
References
- Dollinger, Bryan R., et al. "Reactive oxygen species shielding hydrogel for the delivery of adherent and nonadherent therapeutic cell types." Tissue Engineering Part A 23.19-20 (2017): 1120-1131.
- Jia, Hong, et al. "The tumor cell‐secreted matricellular protein WISP1 drives pro‐metastatic collagen linearization." The EMBO Journal (2019).
- Pérez Cardona, David José. "Phenotype analysis of dermal-epidermal organotypics made with hacat cell line seeding on top of three human fibroblast populated matrices (polyethylene terephthalate, fibrin or collagen I+ III)." (2017).
- Karki, Surya B., et al. "Investigation of non-thermal plasma effects on lung cancer cells within 3D collagen matrices." Journal of Physics D: Applied Physics 50.31 (2017): 315401.
- Keating, M., et al. "Spatial distributions of pericellular stiffness in natural extracellular matrices are dependent on cell-mediated proteolysis and contractility." Acta biomaterialia 57 (2017): 304-312.
- Blum, Kevin M., et al. "Acellular and cellular high-density, collagen-fibril constructs with suprafibrillar organization." Biomaterials science 4.4 (2016): 711-723.
- Kaufman, Gili, and Drago Skrtic. "Spatial development of gingival fibroblasts and dental pulp cells: Effect of extracellular matrix." Tissue and Cell 49.3 (2017): 401-409.