CytoSoft® Imaging 96-well Plate, 2 kPa

Cat.-Nr.: 5257-1EA

Description

CytoSoft®-Imaging products provide a tool to culture cells on PDMS substrates with various rigidity. This CytoSoft®-Imaging plate comes with 1 glass-bottom 96-well plate for high resolution imaging of cells.

This CytoSoft® Imaging 96-well product has a defined elastic modulus (see certificate of analysis) with a glass bottom for high resolution imaging The thickness of the silicone gel is uniform with a ~0.03 mm thick layer of silicone in each well. The silicone gels are activated and ready to bind to a purified ECM, such as PureCol® type I collagen (#5005) prior to cell addition.
The rigidity of the substrate to which cells adhere can have a profound effect on cell morphology and gene expression. CytoSoft® products provide a tool to culture cells on substrates with various rigidities covering a broad physiological range using a thin layer of biocompatible silicone.

The CytoSoft® – Imaging plate has a glass bottom for high-resolution imaging where low autofluorescence and exceptional optical clarity are required. The 96-well format is optimal for high-throughput cell studies and screening. The plate consists of a #1.5 thickness glass bottom bonded to a black polystyrene frame and includes a lid. The plates are sterilized using ozone and provided with 1 plate per package.

On the bottom of each well, there is a thin layer of specially formulated biocompatible silicone, whose elastic modulus (rigidity) is carefully measured and certified. The surfaces of the gels in CytoSoft® products are functionalized to form covalent bonds with amines on proteins. The chemical functionalization is stable and the reaction does not require a catalyst, facilitating coating of the gel surfaces with matrix proteins and plating cells.

The silicone substrates are optically clear and have a near zero auto-florescence. The layer of silicone in each well is firmly bonded to the bottom of the well. Unlike hydrogels (such as polyacrylamide gels), silicone gels are not susceptible to hydrolysis, do not dry or swell, are resilient and resistant to tearing or cracking, and their elastic moduli (rigidities) remain nearly unchanged during extended storage times.
CytoSoft®-Imaging products accommodate live cell staining using membrane and cell permeable dyes; fixation of cells using common techniques such as paraformaldexyde; and immunostaining of fixed cells.

CytoSoft coating reagents:

PureCol® (bovine collagen), Human Type III Collagen Solution, Human Type IV Collagen Solution, Human Fibronectin Solution, Human Vitronectin Solution

  • SUPPLIER:

    Advanced BioMatrix

  • STATUS:

    In Stock

  • SIZE:

    1 pcs

Overview

  • Species: Synthetic
  • Keywords: CytoSoft Substrate (Rigidity) Products
  • Specific Attributes: CytoSoft Imaging
  • Product Type: CytoSoft-Rigidity Plates
  • Features: 2 kPa
  • Packaging:1 x 96-well plate
  • Features:Rigidity (elastic modules) 2 kPa

Related Files

Datasheet
Certificate of Material Origin

References

  • References for CytoSoft® Products:
  • Modaresi, Saman, et al. “Deciphering the Role of Substrate Stiffness in Enhancing the Internalization Efficiency of Plasmid DNA in Stem Cells Using Lipid-Based Nanocarriers.” Nanoscale, vol. 10, no. 19, 2018, pp. 8947–8952., doi:10.1039/c8nr01516c.
  • Wilson, Christina L. In Vitro Models Of Brain For Study Of Molecular Mechanisms In Brain Disorder. The University of Nebraska-Lincoln, 2016.
  • Wilson, C. L., Hayward, S. L., & Kidambi, S. (2016). Astrogliosis in a dish: Substrate stiffness induces astrogliosis in primary rat astrocytes. RSC Advances, 6(41), 34447-34457. doi:10.1039/c5ra25916a
  • Prager-Khoutorsky M, Lichtenstein A, Krishnan R, Rajendran K, Mayo A, Kam Z, Geiger B, Bershadsky AD. Fibroblast polarization is a matrix-rigidity-dependent process controlled by focal adhesion mechanosensing. Nat. Cell Biol. 2011; 13:1457–1465.
  • Gutierrez E, Tkachenko E, Besser A, Sundd P, Ley K, Danuser G, Ginsberg MH, Groisman A. High Refractive Index Silicone Gels for Simultaneous Total Internal Reflection Fluorescence and Traction Force Microscopy of Adherent Cells. PLoS One. 2011; 6:e23807.
  • Merkel R, Kirchgessner N, Cesa CM, Hoffmann B. Cell force microscopy on elastic layers of finite thickness. Biophys. J. 2007; 93:3314–23.
  • Schellenberg, A. et al. Matrix elasticity, replicative senescence and DNA methylation patterns of mesenchymal stem cells. Biomaterials 35, 6351–8 (2014).
  • Cesa, C. M. et al. Micropatterned silicone elastomer substrates for high resolution analysis of cellular force patterns. Rev. Sci. Instrum. 78, 034301 (2007).
  • Gutierrez, E. & Groisman, A.  Measurements of Elastic Moduli of Silicone Gel Substrate with a Micro fluidic Device. Plos One 6 (2011).
  • Mori, H., Takahashi, A., Horimoto, A., and Hara, M. Migration of glial cells differentiated from neurosphere-forming neural stem/progenitor cells depends on the stiffness of the chemically cross-linked collagen gel substrate. Neuroscience Letters, Vol. 555, October (2013)
  • Banerjee, I., Carrion, K., Serrano, R., Dyo, J., Sasik, R., Lund, S. et al. Cyclic stretch of embryonic cardiomyocytes increases proliferation, growth, and expression while repressing Tgf-β signaling. J Mol Cell Cardiol. 2015; 79: 133–144
  • Vertelov, G. et al. Rigidity of silicone substrates controls cell spreading and stem cell differentiation. Sci. Rep. 6, 33411; doi: 10.1038/srep33411 (2016).
  • Tkachenko E, Rawson R, La E, et al. Rigid Substrate Induces Esophageal Smooth Muscle Hypertrophy and EoE Fibrotic Gene Expression. The Journal of allergy and clinical immunology. 2016;137(4):1270-1272.e1. doi:10.1016/j.jaci.2015.09.020.
  • Sao, K. et al. Myosin II governs intracellular pressure and traction by distinct tropomyosin-dependent mechanisms. Molecular Biology of the Cell30,1170–1181 (2019).
  • Cooper, J. G. et al. Spinal Cord Injury Results in Chronic Mechanical Stiffening. Journal of Neurotrauma (2019). doi:10.1089/neu.2019.6540

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