Overview
- Species: Rat
- Keywords: Neurotrophins
- Product Type: Ciliary-Neurotrophic Factor
- Donor / Source:Escherichia Coli.
- Formulation:Lyophilized from a concentrated (1mg/ml) solution in water containing 0.025% NaHCO3.
- Purity:Greater than 99.0% as determined by: (a) Analysis by Gel Filtration. (b) Analysis by SDS-PAGE.
- Amino acid sequence:AFAEQTPLTL HRRDLSSRSI WLARKIRSDL TALMESYVKH QGLNKNINLD SVDGVPVAST DRWSEMTEA ERLQENLQAY RTFQGMLTKL LEDQRVHFTP TEGDFHQAIH TLMLQVSAFA YQLEELMVLL EQKIPENEADG MPATVGDGG LFEKKLWGL KVLQELSQWT VRSIHDLRV ISSHQMGISA LESHYGAKDK QM
- Solubility:It is recommended to reconstitute the lyophilized CNTF in sterile water or 0.4% NaHCO3 adjusted to pH 8-9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein.
- Stability:Lyophilized CNTF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CNTF should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
