Overview
- Species: Human
- Keywords: Growth Factors
- Product Type: Colony Stimulating Factor
- Donor / Source:Escherichia Coli.
- Formulation:GCSF was lyophilized after extensive dialysis against 10mM sodium acetate buffer pH= 4.
- Purity:Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
- Amino acid sequence:The sequence of the first five N-terminal amino acids of GCSF was determined and was found to be Met-Thr-Pro-Leu-Gly.
- Solubility:It is recommended to reconstitute the lyophilized GCSF in sterile 20mM AcOH not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
- Stability:Lyophilized GCSF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GCSF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
