Overview
- Species: Rabbit
- Keywords: Cytokines
- Product Type: Tumor Necrosis Factor
- Donor / Source:Escherichia Coli.
- Formulation:TNF-alpha Rabbit was lyophilized after extensive dialysis against 20mM PB, pH7.4, 300mM NaCl.
- Purity:Greater than 95% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
- Amino acid sequence:The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ser-Ala-Ser-Arg.
- Solubility:It is recommended to reconstitute the lyophilized Tumor Necrosis Factor-alpha in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
- Stability:Lyophilized Tumor Necrosis Factor-a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TNF-a should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
