TARGATT Technology
TARGATT™ Large Knock-in Technology for Mammalian Cell Line Development
TARGATT™ Technology is a site-specific recombination system engineered for efficient, stable, and single-copy insertion of large gene fragments into defined mammalian safe harbor loci (such as the H11 locus). Unlike traditional viral or CRISPR-based knock-in methods, TARGATT™ utilizes a site-specific integrase and pre-engineered landing pad cell lines, overcoming common challenges associated with random integration and double-strand break-induced off-target effects.
Key Technological Advantages & Scientific Benefits
- Large Cargo Capacity (>50 kb): Accommodates large donor constructs – ranging from single genes to multi-gene circuits exceeding 50 kb – making it ideal for complex protein co-expression, metabolic pathway engineering, and large antibody formats.
- Defined Single-Copy Integration: Delivers precisely one gene copy per cell at a well-characterized genomic safe harbor locus (H11), ensuring predictable, and long-term stable expression without copy-number variability.
- High Knock-In Efficiency (Up to 100%): Reaches high knock-in efficiency post-selection, enabling rapid pool-based screening and high-throughput mammalian display libraries (up to 107–109 complexity) without extensive clonal isolation.
Negligible Off-Target Activity & Unidirectional Insertion: Eliminates double-strand break (DSB) risks and prevents cassette excision, providing a safer alternative to CRISPR and Cre/Flp-mediated recombination systems.- Accelerated Timelines (4–6 Weeks): Streamlines custom mammalian cell line engineering (including HEK293, CHO, and hiPSCs) for difficult-to-express proteins, membrane receptors, and inducible expression systems.
- Clear IP for Commercialization: Fully defined intellectual property landscape suited for basic research, bioproduction, and therapeutic cell therapy applications.
Primary Applications
- Protein & Antibody Engineering: Stable expression of challenging membrane proteins, bispecific antibodies, and multi-protein complexes at defined stoichiometries.
- Mammalian Display & High-Throughput Screening: High-capacity library screening directly in human cells for biologically relevant hits.
- Cell & Gene Therapy Development: High-efficiency safe-harbor gene editing in hiPSC lines and GMP-grade cell systems for scalable disease modeling and translational iPSC research.
For detailed information on TARGATT™ Technology and its applications,
– download the TARGATT™ Technology e-book or
– contact our experts to discuss your specific project needs.
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ActiCells™ GMP-Matching RUO TARGATT™ hiPSC Knock-in Kit
Cat.-Nr: AST-9450
Accelerate development of engineered iPSC-derived cell products with a RUO hiPSC line built for site-specific gene insertion using... Read More