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Home |Products|Matrix Proteins / Bioprinting|Adhesion Molecules & Peptides|Poly-D-Lysine Hydrobromide, Solution

Poly-D-Lysine Hydrobromide, Solution

Cat.-Nr.: 5049-50ML

Description

Poly-D-Lysine is a synthetic amino acid chain that is positively charged and widely used as a coating to enhance cell attachment and adhesion to both plasticware and glass surfaces. This molecule is resistant to enzymatic degradation and has been used to culture a wide variety of cell types.

The molecular weight of Poly-D-Lysine can vary significantly with lower molecular weight (30,000 Da) being less viscous and higher molecular weight (>300,000 Da) having more binding sites per molecule. This product’s molecular weight ranges from 70,000 to 150,000 Da yielding a solution viscosity for easy handling while providing sufficient binding sites for cell attachment.

Poly-D-Lysine surface coatings are designed to improve cell attachment, growth and differentiation of many cell types. Coated surfaces will often improve cell attachment in reduced or serum-free conditions. Poly-D-Lysine is supplied in a sterile 5 mg package size.

Poly-D-Lysine is ideally suited for the coating of coverslips and other glass or plastic cell culture surfaces. Click here for a brief protocol on how to coat your cell culture system.

  • SUPPLIER:

    Advanced BioMatrix

  • STATUS:

    In Stock

  • SIZE:

    50 ml

  • Overview
  • Related Files
  • References

Overview

  • Species: Synthetic
  • Keywords: Coating
  • Specific Attributes: Adhesion peptides
  • Features: animal-free, filtered (22 µm), solution, xeno-free
  • Features:0,1 mg/ml

Related Files

Datasheet
Certificate of Origin Coating Protocol Poly-D-Lysine

References

  • Albuquerque, Cristóvão, et al. "Preparation of coverslips for neuronal cultures." Cold Spring Harbor Protocols 2009.8 (2009): pdb-prot5272.
  • Unsain, Nicolas, et al. "Production and isolation of axons from sensory neurons for biochemical analysis using porous filters." JoVE (Journal of Visualized Experiments) 89 (2014): e51795.
  • Li, Ling, Mizuho Fukunaga-Kalabis, and Meenhard Herlyn. "Isolation, characterization, and differentiation of human multipotent dermal stem cells." Skin Stem Cells. Humana Press, Totowa, NJ, 2013. 235-246.
  • Baik, Matthew, et al. "Identification of invadopodia by TKS5 staining in human cancer lines and patient tumor samples." MethodsX 6 (2019): 718-726.
  • Tammia, Markus, et al. "Egr2 overexpression in Schwann cells increases myelination frequency in vitro." Heliyon 4.11 (2018): e00982.
  • Boularaoui, Selwa Mokhtar, et al. "Efficient transdifferentiation of human dermal fibroblasts into skeletal muscle." Journal of tissue engineering and regenerative medicine 12.2 (2018): e918-e936.
  • Johnstone, Aaron D., et al. "Developmental axon degeneration requires TRPV1-dependent Ca2+ influx." eNeuro 6.1 (2019).

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