26 Assays Using PureCol Collagen

Collagen is the most abundant protein in the body and is thus the most widely used ECM protein in research across hundreds of various assays.

PureCol® Type I Collagen is the most popular collagen product, being 3 mg/ml of type I atelocollagen (>99.9% pure), and is the product referenced in each of the assays below. Other ABM Collagen products may be substituted in these assays if different mechanical properties of the collagen are desired (ie. use Telocollagen for stiffer hydrogels to evaluate changes in migration related to stiffness). Descriptions of some of the assays are found below the chart.

Assay Title	Reference
Boyden Chamber	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8926928/
Spheroid Invasion	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8926928/
Single Cell Motility	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8926928/
Cell Viability	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8991007/
Aortic Ring	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8907187/
3D Chemotaxis	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8836243/
Cell Line Generation	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8812522/
Collagen Migration	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8751638/
Cell Proliferation	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8858314/
Air-Liquid Interface	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8651658/
Gel Contraction	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8556235/
3D Migration	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8523719/
Tumor-On-Chip 3D Motility	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8534269/
In Vitro Granuloma Models	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8486295/
Injectable Gels	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8438067/
3D Cytotoxicity co-culture	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8410835/
Invasion	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8383389/
CRT (Calreticulin)	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8436107/
Cell Binding	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8195475/
Melanoma Spheroid Invasion	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8115293/
Stiffness	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8428240/
LH Enzymatic Activity	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8055892/
In Vitro 3D Cyst	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8251518/
Scratch/Wound Healing	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7830896/
Tube Formation	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7830896/
Permeability	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6873778/

(published 01/26/23: Advanced BioMatrix – 26 Assays Using PureCol Collagen)