iPSC-Derived Natural Killer (NK) Cell Kit (iPSCs from Fibroblasts, iPSC Line ASE-9211, African-American Male, Neonatal)

Description

NK cells are differentiated from an integration-free, control human iPSC line (#ASE-9211), reprogrammed from fibroblasts of an African-American neonatal male donor. These cryopreserved, high-purity cells express high levels of NK cell biomarkers, CD56 and CD45. The differentiated NK cells recapitulate the phenotype and functional parameters of primary and in vivo NK cells.

Scale-up screening and conduct cell-based assays using NK cells with a uniform genetic background with ASE-9285-1, passage 0 (P0) iPSC-derived NK cells.

These cells are derived from ASE-9211, our control iPSC cell line reprogrammed from normal male fibroblasts using an episomal method.

Quality control assays
Each lot of ASE-9285-1 meets the following standards before release

• ≥ 80% purity using flow cytometry and NK cell markers CD45 and CD56
• Each lot tested growth, viability, and the absence of mycoplasma and pathogens

Kit Components (cells and optimized media):

• 1 vial of P0 iPSC-derived NK cells, ≥1 x 10⁶ cells/ vial
• 100 mL of NK Cell Basal Culture Media (for cytotoxicity assays only, at this time)
• 1 mL of NK Cell Culture Media Supplement A (100x)

The kit was formerly available under Catalogue Number ASE-9708.

• SUPPLIER:

  Applied StemCell

• STATUS:

  In Stock

• SIZE:

  1.000.000 cells/vial