Phosphodiesterase I -VPH -(100 U)

Cat.-Nr.: LS003926

Example image of a clear Eppendorf reaction tube with screw cap containing enzyme for research; label is generic

Description

Venom exonuclease (Phosphodiesterase I) successively hydrolyzes 5′-mononucleotides from 3′-OH-terminated ribo- and deoxyribo-oligonucleotides. The enzyme has an optimal pH range of 9.8-10.4 and a molecular weight of 115 kDa. Phosphodiesterase is inhibited by reducing agents such as glutathione, cysteine and ascorbic acids. It is completely inhibited by 5mM EDTA while ATP, ADP and AMP are partial inhibitors. The enzyme has an absolute requirement for Mg2+.
Purified by the method of Williams, Sung and Laskowski, JBC, 236, 1130 (1961). Further treated to inactivate contaminating 5′-nucleotidase activity according to Sulkowski and Laskowski: BBA, 240, 443 (1961). Lyophilized in vials.

  • SUPPLIER:

    Worthington Biochemical

  • STATUS:

    In Stock

  • SIZE:

    100 U

Overview

  • Species: Snake
  • Product Type: Phosphodiesterases
  • Features: Chromatographically purified, lyophilized
  • Donor / Source:Crotalus adamanteus Venom
  • Activity:≥20 units per mg dry weight
  • Code:VPH

Related Files

Sample Certificate of Analysis (CoA)

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