Trypsin, TPCK, Irradiated – TRTVMF

Description

For applications requiring virus free trypsin, Worthington offers VMF (Virus and Mycoplasma Free) Trypsin which is a specially processed trypsin. The product is irradiated to eliminate microorganisms and is quality control tested specifically against Bovine Diarrhea virus, Infectious Bovine Rhinotracheitis (IBR) virus, Herpes virus, Enterovirus, Adenoviruses, Bovine syncytial virus, Parainfluenza type 3 (PI3-SF) and Parvovirus. Additionally, the material is tested to be free of mycoplasma. As a result of the purification process, extraneous proteases and nucleases which could be harmful to cell lines are removed. The trypsin specific activity is very high allowing lower working concentration (0.01% range) resulting in less cell damage.
Trypsin is a pancreatic serine protease with substrate specificity based upon positively charged lysine and arginine side chains. The molecular weight of trypsinogen is 24,000 daltons and 23,800 daltons for trypsin. The optimum pH is 8.0. Trypsin is inhibited by organophosphorus compounds such as diisopropyl fluorophosphate (DFP) and natural inhibitors from pancreas. Soybean, lima bean, and egg white are also sources of natural inhibitors.
Chromatographically purified trypsin treated with L-(tosylamido-2-phenyl) ethyl chloromethyl ketone (TPCK) to inhibit contaminating chymotryptic activity according to Kostka, V., and Carpenter, F.H.: JBC, 239, 1799 (1964), Code: TRTPCK, lyophilized, irradiated and tested for the absence of mycoplasma and extraneous virus according to 9 CFR 113.53c.
An irradiated form of our TPCK-treated trypsin is also available. See product code TRTVMF.

• SUPPLIER:

  Worthington Biochemical

• STATUS:

  In Stock

• SIZE:

  100 mg