Ribonuclease A, DNase & Protease Free – RPDF (1 mg)

Description

Pancreatic ribonuclease (RNase I) catalyzes cleavage of the phosphodiester bond between the 5′-ribose of a nucleotide and the phosphate group attached to the 3′-ribose of an adjacent pyrimidine nucleotide forming a 2′,3′-cyclic phosphate which may then be hydrolyzed to the corresponding 3′-nucleoside phosphate. Ribonuclease A has a molecular weight of 13,700 daltons. It operates in an optimum pH range of 7.0-7.5.
Ribonuclease is inhibited by heavy metal ions and it is competitively inhibited by DNA. Since Molecular Biology Grade RNase A (Product Code: RPDF) is essentially free of DNase and protease activities, this product is useful in removing RNA from DNA in nucleic acid work and where other enzymes are used or where intact proteins must be recovered.
Ribonuclease A, DNase & Protease Free (Product Code: RPDF) is suitable as supplied for applications requiring minimal DNase and protease levels and needs no further treatment.

Molecular Biology Grade. A solution containing approximately 5 mg/ml in 50% glycerol. Prepared specifically for use in purifying DNA plasmids. Each lot is assayed for DNase and protease.

• SUPPLIER:

  Worthington Biochemical

• STATUS:

  In Stock

• SIZE:

  1 mg