Ribonuclease U2, Recombinant, Animal-free

Ustilago sphaerogena ribonuclease U2 produced recombinantly in yeast (Komagataella phaffi-previously P. pastoris) to decrease contaminant nucleases and proteases and eliminate potential pathogens associated with animal-sourced materials.

Ribonuclease U2 is an endonuclease that hydrolyzes the phosphodiester bonds of 3’-linked ribonucleotides, resulting in nucleoside 3’-phosphates and 3’-phosphooligonucleotides ending in Ap or Gp with 2’, 3’-cyclic intermediates. It is purine selective with a preference for adenosine and has the following order of cleavage specificity (A>G>C>U). Because of this, it is helpful in RNA sequencing and RNA modification and mapping applications. Optimal activity is observed at pH 4.5.

Unit Definition