Overview
- Species: Human
- Product Type: Platelet & other Plasma Proteins
- Formulation:20mM Hepes, 150mM NaCl, pH 7.4, 10 mM CHAPS
Human Tissue Factor, Native, Placental
Cat.-Nr.: HTF-0310
Description
Tissue factor (TF) is a transmembrane protein that functions as the high affinity receptor for factor VIIa (FVIIa)1. It is “sequestered” on the surface of cells not exposed to blood. Following vascular injury, formation of a TF/FVIIa complex (aka extrinsic tenase complex) initiates the hemostatic response by catalyzing the conversion of factor X to factor Xa and factor IX to factor IXa. Beyond its role in coagulation, evidence of a role for TF/FVIIa in cell signalling responses has also been observed2,3.
TF consists of three domains4: (1) an extracellular domain, that serves as a template for factor VIIa binding; (2) a transmembrane domain; and (3) a cytosolic domain of 21 amino acids length inside the cell which is involved in the signaling function of TF. Soluble forms of TF lacking the transmembrane and cytoplasmic domains have been observed though their roles in promoting thrombin formation are not clear5.
Native Human (Placental) Tissue Factor (HTF) is purified from human placental tissue using conventional chromatography techniques. Purified HTF is supplied in 20 mM Hepes, 0.15 M NaCl, pH 7.4 containing 10 mM CHAPS and should be stored at -80°C. Purity is determined by SDS-PAGE analysis and activity measured using a fluorogenic substrate.
SUPPLIER:
Prolytix
STATUS:
In Stock
SIZE:
10 µg
Native Human Placental Tissue Factor, purified from human placental tissue using chromatography techniques.
References
-
Mackman N, Tilley RE, Key NS. Role of the extrinsic pathway of blood coagulation in hemostasis and thrombosis. Arteriosclerosis, Thrombosis, and Vascular Biology. 2007 Aug;27(8):1687-1693. DOI: 10.1161/atvbaha.107.141911. PMID: 17556654.
- Ruf W, Disse J, Carneiro-Lobo TC, Yokota N, Schaffner F. Tissue factor and cell signalling in cancer progression and thrombosis. J Thromb Haemost. 2011 Jul;9 Suppl 1(Suppl 1):306-15. doi: 10.1111/j.1538-7836.2011.04318.x. PMID: 21781267; PMCID: PMC3151023.
- Eriksson O, Ramström M, Hörnaeus K, Bergquist J, Mokhtari D, Siegbahn A. The Eph tyrosine kinase receptors EphB2 and EphA2 are novel proteolytic substrates of tissue factor/coagulation factor VIIa. J Biol Chem. 2014 Nov 21;289(47):32379-91. doi: 10.1074/jbc.M114.599332. Epub 2014 Oct 3. PMID: 25281742; PMCID: PMC4239594.
- Muller YA, Ultsch MH, de Vos AM. The crystal structure of the extracellular domain of human tissue factor refined to 1.7 A resolution. J Mol Biol. 1996 Feb 16;256(1):144-59. doi: 10.1006/jmbi.1996.0073. PMID: 8609606.
- Bogdanov VY, Versteeg HH. "Soluble Tissue Factor" in the 21st Century: Definitions, Biochemistry, and Pathophysiological Role in Thrombus Formation. Semin Thromb Hemost. 2015 Oct;41(7):700-7. doi: 10.1055/s-0035-1556049. Epub 2015 Sep 26. PMID: 26408917; PMCID: PMC4827326.
Rat Factor X