Overview
- Species: Human
- Product Type: Platelet & other Plasma Proteins
Human Lyophilized Phospholipid Vesicles
Cat.-Nr.: PCPS-02
Description
For the enzymatic reactions of the coagulation cascade to occur at physiologically relevant rates, the components must be localized to an appropriate membrane surface comprised of negatively-charged phospholipids.1 Activated platelets2,3 and to a lesser extent mononuclear cells4 provide this membrane surface in vivo, whereas synthetic phospholipid vesicles composed of 75% phosphatidylcholine (PC) and 25% phosphatidylserine (PS) (PCPS) can support these reactions in vitro.6
PCPS vesicles are prepared from L-α-phosphatidylcholine (chicken egg) and L-α-phosphatidylserine (porcine brain)6 and quantified by phosphate assay.7 They are provided as a lyophilizate and should be stored at 4°C prior to and following reconstitution.
SUPPLIER:
Prolytix
STATUS:
In Stock
SIZE:
5 µMol
References
- Mann K, Nesheim M, Church W, Haley P, Krishnaswamy S. Surface dependent reactions of the vitamin K dependent enzyme complexes. Blood. 1990;76:1–16.
- Chandler A. An overview of thrombosis and platelet involvement in the development of the human atherosclerotic plaque. In: Glagov S, Newman W, Schaffer S, eds. Pathobiology of the Human Atherosclerotic Plaque. New York, NY: Springer-Verlag; 1990:359–378.
- White J. Anatomy and structural organization of the platelet. In: Coman R, Hirsh J, Marder V, Salzman E, eds. Hemostasis and Thrombosis: Basic Principles and Clinical Practice Philadelphia, Pa: JB Lippincott Co; 1994:397–413.
- Tracy P, Allen D, Worfolk L, Robinson-Lawler R. Monocyte/macrophage regulation of coagulation events. Hemostasis. 1996;26(suppl 1):6–11.
- Nesheim M, Taswell J, Mann K. The contribution of bovine factor V and factor Va to the activity of prothrombinase. J Biol Chem. 1979;254:10952–10962.
- Barenholz Y, Gibbs D, Litmann B, Goll J, Thompson E, Carlson F. A simple method for the preparation of homogeneous phospholipid vesicles. Biochemistry. 1977;16:2806–2810.
- Gomori G. A modification of the colorimetric phosphorous determination for use with a photoelectric colorimeter. J Lab Clin Med. 1942;27:955–960.
Rat Factor X