Description
The CD3+ Pan T Cell 5M System Complete Kit includes:
Human CD3+ Pan T Cells with 5 x 106 cells per vial and RPMI 1640-FBS Medium Complete Kit with frozen basal medium containing phenol red, 2 mM L-glutamine, 25 mM HEPES and 1 mM sodium pyruvate. The Kit also contains Fetal Bovine Serum and Gentamicin Amphotericin B.
Human CD3+ Pan T Cells are cryopreserved directly after isolation to ensure optimal phenotype and the highest viability. Human CD3+ Pan T Cells are quality tested via flow cytometry to ensure proper expression of multiple markers of various hematopoietic lineage cells.
CD3+ Pan T Cells have not been exposed to antimicrobials or phenol red. Although antimicrobials are not required for eukaryotic cell proliferation, they are recommended to inhibit potential fungal or bacterial contamination of eukaryotic cell cultures.
Human CD3+ Pan T Cells are cryopreserved at 10 x 106 cells per vial.
Normal Human CD3+ Pan T Cells are tested for:
- Cell Count:
- ≥ 5 x 106 Human CD3+ Pan T Cells per vial
- Cell Viability:
- Minimum 70% viability when thawed from cryopreservation
- Sterility Testing:
- Negative for mycoplasma, bacterial and fungal growth
- Virus Testing:
- Negative for HIV-1, HIV-2, HBV, and HCV by PCR
- Flow Cytometry Analysis:
- Tested for CD3, CD4, and CD8
- > 80% positive for CD3
For an overview of available hematopoietic cells and isolated subsets from all available sources (leukapheresis, periperal blood, cord blood, bone marrow and iPSC-derived), see the product list: Human Hematopoietic Cells (click).