Overview
- Species: Synthetic
- Keywords: HyStem®/hyaluronic acid
- Specific Attributes: sterile
Extralink® PEGDA
Cat.-Nr.: GS3006-5EA
Description
Extralink® (PEGDA, polyethylene glycol diacrylate) 5 x 2.5mL vials of diacrylated PEG . Vials are blanketed by nitrogen and under a slight vacuum.
Extralink® is polyethylene diacrylate (PEGDA) with a molecular weight of 3500 Da. It is a thiol-reactive crosslinker that convalently reacts with the thiol groups in Glycosil®, Heprasil® and Gelin-S® to form viscoelastic hydrogels. Extralink® vials are >80% acrylated and supplied as the crosslinking component in all HyStem® hydrogel kits.
Extralink® vials are paired with HyStem-C and HyStem–HP kits while Extralink-Lite vials are paired with HyStem hydrogel kits to provide comparable experiences despite the different HA concentrations.
Gelation
Extralink® is used to chemically crosslink Glycosil (or Heprasil) and Gelin-S in Hystem-C and HyStem-HP kits. The gelation time ranges from as short as 5 minutes to as long as a couple of hours, depending upon the amount of Extralink used and the concentration/dilution of the Glycosil, Gelin-S and/or Heprasil.
Similarly, Extralink-Lite is used in place of Extralink for applications where Gelin-S is not included. The higher relative concentration of Glycosil requires less crosslinker to emulate the gelation time and stiffness of HyStem-C and HyStem-HP.
SUPPLIER:
Advanced BioMatrix
STATUS:
In Stock
SIZE:
5 x 2,5 ml
References
- References for HyStem®:
- Maloney, E. et al.Immersion Bioprinting of Tumor Organoids in Multi-Well Plates for Increasing Chemotherapy Screening Throughput. Micromachines 11, 208 (2020).
- Gaetani, R., et al. (2015) Epicardial application of cardiac progenitor cells in a 3D-printed gelatin/hyaluronic acid patch preserves cardiac function after myocardial infarction. Biomaterials 61: 339-348. PMID: 17335875.
- Prestwich, G.D., et al. (2007) 3-D culture in synthetic extracellular matrices: new tissue models for drug toxicology and cancer drug discovery. Adv Enzyme Regul 47: 196-207. PMID: 17335875.
- Shu, X.Z., et al. (2006) Synthesis and evaluation of injectable, in situ crosslinkable synthetic extracellular matrices for tissue engineering. J Biomed Mater Res A 79: 901-912. PMID: 16941590.
- Shu, X.Z., et al. (2003) Disulfide-crosslinked hyaluronan-gelatin hydrogel films: a covalent mimic of the extracellular matrix for in vitro cell growth. Biomaterials 24: 3825-3834. PMID: 12818555.
- Cai, et al. (2005) Injectable glycosaminoglycan hydrogels for controlled release of human basic fibroblast growth factor.Biomaterials, 26, 6054-6067.
- B. Pike, et al. (2006) Heparin-regulated release of growth factors in vitro and angiogenic response in vivo to implanted hyaluronan hydrogels containing VEGF and bFGF. Biomaterials, 27, 5242–5251.
- D. Prestwich, et al. (2007) 3-D Culture in Synthetic Extracellular Matrices: New Tissue Models for Drug Toxicology and Cancer Drug Discovery. invited, Adv. Enz. Res., in press (2007).
- Z. Shu, et al, (2006) Synthesis and Evaluation of Injectable, In Situ Crosslinkable Synthetic Extracellular Matrices (sECMs) for Tissue Engineering. J. Biomed Mater. Res. A, 79A(4), 901-912.
- Shu, X.Z., et al. (2004) In situ crosslinkable hyaluronan hydrogels for tissue engineering. Biomaterials 25: 1339-1348. PMID: 14643608.
- Mehra, T.D., et al. (2006) Molecular stenting with a crosslinked hyaluronan derivative inhibits collagen gel contraction. J Invest Dermatol 126: 2202-2209. PMID: 16741511.
- Shu, X.Z., et al. (2004) Attachment and spreading of fibroblasts on an RGD peptide-modified injectable hyaluronan hydrogel. J Biomed Mater Res A 68: 365-375. PMID: 14704979.
- Ghosh, K., et al. (2007) Cell adaptation to a physiologically relevant ECM mimic with different viscoelastic properties. Biomaterials 28: 671-679. PMID: 17049594.
Lifeink® 200; Type I Collagen Bioink