DNase I, RNase & Protease free – DPRF (2500 U)

Description

Bovine pancreatic deoxyribonuclease is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with free hydroxyl group at the 3′ position and phosphate group at the 5′ position. The average chain length of a limit digest is a tetranucleotide.

Uses: Worthington offers DNase at different levels of purity for different applications. Product Codes: DPRF (and DPRFS) are both especially designed for Molecular Biology applications and contain the lowest levels of RNase and protease activity. They are both suitable for use in techniques requiring digestion of DNA in the recovery of intact RNA or where the integrity of structural proteins or enzymes must be maintained. Applications have included nick translation, DNA mapping, isolation of nuclear RNA and protein, plasmid construction, and RNA polymerase synthesis of RNA probes and RT-PCR.

DNase I, RNase & Protease free – DPRF (2500 U) has Molecular Biology Grade. Chromatographically purified to remove RNase and protease. Lyophilized in vial containing glycine and calcium as stabilizer. Dissolving the entire 10,000 unit vial in 5 ml, or the entire 2,500 unit vial in 1.25 ml, provides the equivalent of a 1 mg/ml solution. (ku = 1000un)

• SUPPLIER:

  Worthington Biochemical

• STATUS:

  In Stock

• SIZE:

  2500 U