Tissue Dissociation Kits
Tissue Dissociation Kits: Tailored for Specialized Tasks
The ready-to-use Tissue Dissociation Kits have been developed to provide researchers with reliable, reproducible, and robust cell isolation systems. The kits feature carefully selected purified enzymes as well as wellproven protocols. The enzymes are lot tested to minimize variations, assure performance, and maximize yield and viability of isolated cells.
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Therefore, ready-to-use Tissue Dissociation Kits offer a cost-effective solution for tissue dissociation. In contrast, the use of crude enzyme preparations with lot-variable enzymatic activities, may require pre-screening of enzyme lots and a continuous modification of isolation parameters and adaption protocols.
Ready-to-use Tissue Dissociation Kits
We offer ready-to-use Tissue Dissociation Kits for the following tissues:
- Papain Dissociation System (Code: PDS) for Neural Tissue Dissociation: Isolation of morphologically intact cortical neurons (nerve cells) from postnatal rat hippocampus, other fetal and postnatal brain regions.
- Hepatocyte Isolation System (Code: HIS) for Liver Tissue Dissociation: Isolation of hepatocytes (liver cells) from adult rat livers.
- Neonatal Cardiomyocyte Isolation System (Code: NCIS) for Heart Tissue Dissociation: Isolation of neonatal cardiomyocyte (heart cells) from neonatal rat heart.
- Cell Isolation Optimizing System (Code: CIS) as a complete Method Development Kit. It acts as a ‘Starter Kit’ for developing optimized cell isolation protocols to meet individual requirements. The Cell Isolation Optimizing System offers purified enzymes commonly used in tissue dissociation, along with recommendations, references, and strategies for optimizing cell isolation procedures cost-effectively. It includes enzymes referenced in tissue dissociation and cell isolation protocols, along with a guide detailing tissue types, enzyme modes of action, tissue culture techniques, and optimization guidelines for successful enzymatic cell isolation.
The kit components of all ready-to-use Tissue Dissociation Kits are also available separately.
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Papain Dissociation System (PDS), 1 Kit
Cat.-Nr: LK003150
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's balanced salt solution (EBSS), and an inhibitor vial for... Read More
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Papain Dissociation System w/o EBSS (PDS2), 1 Kit
Cat.-Nr: LK003160
Complete kit as described for product Code: PDS, but without the Earle's Balanced Salt Solution (EBSS). LK003160 contains the following... Read More
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CELSTRNK - Cell Strainers, Falcon, NCIS Kit
Cat.-Nr: LK003265
Cell strainers (Falcon), components of the NCIS kit. Suitable for removal of tissue debris in cell isolation applications.
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CIT - Cell Isolation Optimizing System
Cat.-Nr: LK003200
A complete method development kit containing an assortment of enzymes most frequently used in tissue dissociation and cell isolation procedures.... Read More
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CLSH - Collagenase/Elastase
Cat.-Nr: LK002066
Enzyme vial of HIS kit that contains 22,500 units of collagenase (Code: CLS-1) and 30 units of elastase (Code: ESL), filtered through 0.22 micron... Read More
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CLSH - Collagenase/Elastase
Cat.-Nr: LK002067
Enzyme vial of HIS kit that contains 22,500 units of collagenase (Code: CLS-1) and 30 units of elastase (Code: ESL), filtered through 0.22 micron... Read More
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CLSPANK - CLSPA Vial, 0.22 Filtered, NCIS
Cat.-Nr: LK003240
A component of the NCIS kit. This material is 0.22 micron membrane filtered and lyophilized in autoclaved vials. A vial reconstituted with 5 ml of... Read More
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CLSPANK - CLSPA Vial, 0.22 Filtered, NCIS
Cat.-Nr: LK003245
A component of the NCIS kit. This material is 0.22 micron membrane filtered and lyophilized in autoclaved vials. A vial reconstituted with 5 ml of... Read More
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D2 - Dnase 2 - PDS Kit
Cat.-Nr: LK003170
A component of the Papain Dissociation System. This material is 0.22 micron membrane filtered and lyophilized in autoclaved vials. A vial... Read More
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D2 - DNase 2 - PDS-Kit
Cat.-Nr: LK003172
A component of the Papain Dissociation System. This material is 0.22 micron membrane filtered and lyophilized in autoclaved vials. A vial... Read More
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EBSS - Earle's Bal. Salt Sol. Vial PDS-Kit
Cat.-Nr: LK003188
Sterile Earle’s Balanced Salt Solution (EBSS) with calcium, magnesium, bicarbonate and phenol red, 1 x 100ml per package, as supplied in the Papain... Read More
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HBSS - Hank's Balanced Salt Solution
Cat.-Nr: LK003210
Sterile calcium and magnesium free Hank's balanced salt solution (CMFHBSS), pH 7.4, as supplied in the NCIS kit; 1 x 500 ml.
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HBSS10 - Hank's Balanced Salt Solution 10x
Cat.-Nr: LK002064
Sterile calcium and magnesium free Hank's balanced salt solution, 10X CMF-HBSS concentrate, as supplied in the HIS kit; 1 x 500 ml
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HIS - Hepatocyte Isolation System
Cat.-Nr: LK002060
Kit for performing five separate adult rat liver perfusions. Contains five single use CLSH enzyme vials, five single use DNase vials, 10X CMF-Hank's... Read More
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L15NK - Leibovitz L15 Media,NCIS
Cat.-Nr: LK003250
Leibovitz L-15 media powder, a component of the NCIS kit. Reconstitute entire contents of pouch, QS to 1 liter with cell culture grade water, and... Read More
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MOPS - 0.15m, MOPS Buffer, (MOPS)
Cat.-Nr: LK002070
0.15M MOPS, pH 7.5, 0.22u filtered. Buffer concentrate ( 75 ml) used to buffer the constituted Leibovitz L-15 media in Hepatocyte Isolation System.
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NAH - Sodium bicarbonate, 7,5%
Cat.-Nr: LK002069
7.5% Sodium bicarbonate concentrate (NaHCO3), used to buffer the diluted CMF-HBSS; 1 x 100 ml
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NCIS - Neonatal Cardiomyocyte Isolation Kit, 1 Kit
Cat.-Nr: LK003300
Kit for performing five separate tissue dissociations each containing up to twelve hearts. Contains single use vials of purified collagenase and... Read More
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NCIS - Neonatal Cardiomyocyte Isolation Kit, 3 Kits
Cat.-Nr: LK003303
Kit for performing five separate tissue dissociations each containing up to twelve hearts. Contains single use vials of purified collagenase and... Read More
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OI-BSA - Inhibitor Vial PDS Kit
Cat.-Nr: LK003182
Ovomucoid protease inhibitor and bovine serum albumin which is 0.22 micron filtered and lyophilized in autoclaved vials to contain 10 mg/ml each upon... Read More
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PAP2 - Papain - PDS-Kit
Cat.-Nr: LK003176
A component of the Papain Dissociation System, for use in the tissue dissociation method of Huettner, J.E., and Baughman, R.W.: J. Neuroscience, 6,... Read More
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PAP2 - Papain - PDS-Kit
Cat.-Nr: LK003178
A component of the Papain Dissociation System, for use in the tissue dissociation method of Huettner, J.E., and Baughman, R.W.: J. Neuroscience, 6,... Read More
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Papain Dissociation System (PDS), 3 Kits
Cat.-Nr: LK003153
This kit consists of 3 x LK003150, which each consists of a set of five single use vials of papain and five single use vials of DNase, 100 ml of... Read More
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Papain Dissociation System w/o EBSS (PDS2), 3 Kits
Cat.-Nr: LK003163
Complete kit as described for product Code: PDS (LK003153), but without the Earle's Balanced Salt Solution (EBSS). This kit consists of 3 x... Read More
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SICNK - Soybean Trypsin Inhibitor, 0.22 Filt.
Cat.-Nr: LK003230
A component of the NCIS kit. This material is 0.22 micron membrane filtered and lyophilized in autoclaved vials. A vial reconstituted with 1 ml of... Read More
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SICNK - Soybean Trypsin Inhibitor, 0.22 Filt.
Cat.-Nr: LK003235
A component of the NCIS kit. This material is 0.22 micron membrane filtered and lyophilized in autoclaved vials. A vial reconstituted with 1 ml of... Read More
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Tissue Guide
Cat.-Nr: TG2020X
Worthington Tissue Dissociation Guide Introduction Tissue dissociation/primary cell isolation and cell harvesting are principal applications... Read More
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TRLSNK - Trypsin Vial, 0.22 Filt., NCIS
Cat.-Nr: LK003220
A component of the NCIS kit. This material is 0.22 micron membrane filtered and lyophilized in autoclaved vials. A vial reconstituted with 2 ml of... Read More
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TRLSNK - Trypsin Vial, 0.22 Filtered, NCIS
Cat.-Nr: LK003225
A component of the NCIS kit. This material is 0.22 micron membrane filtered and lyophilized in autoclaved vials. A vial reconstituted with 2 ml of... Read More