Papain & Trypsin

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Papain & Trypsin

Papain and Trypsin are proteolytic enzymes commonly used for tissue dissociation in various biomedical and research applications.

Papain is a cysteine protease derived from the papaya fruit (Carica papaya). It cleaves

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peptide bonds in proteins, particularly those adjacent to amino acids with large hydrophobic side chains. Papain is widely used in tissue dissociation procedures. Due to its gentle proteolytic activity and broad substrate specificity, it is very effective in breaking down extracellular matrix proteins while preserving the integrity and viability of the cells.
Papain is well suited to be used in combination with other enzymes, like Collagenase, Dispase or Trypsin, depending on experimental needs.

Trypsin is a pancreatic serine protease. It cleaves peptide bonds on the carboxyl side of lysine and arginine residues. Trypsin is commonly used for isolating primary cells from tissues. While purified Trypsin alone is generally ineffective due to its limited selectivity for extracellular proteins, combining it with other enzymes like Elastase or Collagenase has shown effective dissociation.

Trypsin is frequently useded to detach adherent cells from tissue culture surfaces for cell harvesting in the context of cell propagation. Therefore, our product portfolio includes purified Trypsin ( Code: TL, TRL, TRLS, and TRLVMF) and ready-to-use Trypsinization Kits, which are also available xeno-free.

Trypsin inhibitors are commonly used to control the activity of trypsin and other proteolytic enzymes during the tissue dissociation process. Trypsin Inhibitors from soybean or lima bean inactivate trypsin on an equimolar basis. Most widely used is the inhibitor from soybean, which also inhibits Chemotrypsin.  The soybean and lima bean Trypsin Inhibitors are animal free.
Ovomucoids are the glycoprotein protease inhibitors of avian egg white and can also inhibit proteases like trypsin.

DNase is also used in tissue culture work to digest DNA from damaged cells thereby reducing viscosity, and removing membrane bound DNA fragments. Therefore, cell aggregation is prevented. Codes: DP and DCLS are suitable for these applications.
The choice and combination of enzymes depends on the tissue type, experimental requirements, and desired outcomes of the tissue dissociation procedure.

A collection of Tissue Dissociation Protocols for many different tissues from a broad spectrum of species can be found in the Worthingtons’ Tissue Dissociation Guide.

  • LBI - Trypsin Inhibitor, Lima Bean

    Cat.-Nr: LS002829

    Fraction III of the preparation described by Fraenkel-Conrat, et al., Arch. Biochem. Biophys., 37, 393 (1952). Supplied as a dialyzed, lyophilized... Read More

  • LBI - Trypsin Inhibitor, Lima Bean

    Cat.-Nr: LS002830

    Fraction III of the preparation described by Fraenkel-Conrat, et al., Arch. Biochem. Biophys., 37, 393 (1952). Supplied as a dialyzed, lyophilized... Read More

  • OI - Trypsin Inhibitor, Ovomucoid

    Cat.-Nr: LS003085

    Mucoprotein and antitryptic factor of egg white described by Lineweaver and Murray, J. Biol. Chem., 171, 565 (1947). A dialyzed, dried powder.

  • OI - Trypsin Inhibitor, Ovomucoid

    Cat.-Nr: LS003086

    Mucoprotein and antitryptic factor of egg white described by Lineweaver and Murray, J. Biol. Chem., 171, 565 (1947). A dialyzed, dried powder.

  • OI - Trypsin Inhibitor, Ovomucoid

    Cat.-Nr: LS003087

    Mucoprotein and antitryptic factor of egg white described by Lineweaver and Murray, J. Biol. Chem., 171, 565 (1947). A dialyzed, dried powder.

  • PAP - Papain, 100mg

    Cat.-Nr: LS003126

    Supplied as a 2X crystalline suspension in 50mM sodium acetate, pH 4.5. To insure full activity, the enzyme should be incubated in a solution... Read More

  • PAP - Papain, 1g

    Cat.-Nr: LS003127

    Supplied as a 2X crystalline suspension in 50mM sodium acetate, pH 4.5. To insure full activity, the enzyme should be incubated in a solution... Read More

  • PAP - Papain, 25 mg

    Cat.-Nr: LS003124

    Supplied as a 2X crystalline suspension in 50mM sodium acetate, pH 4.5. To insure full activity, the enzyme should be incubated in a solution... Read More

  • PAPL - Papain lyophilized, 1 g

    Cat.-Nr: LS003120

    Supplied as a lyophilized powder prepared from a 2X crystalline suspension, Code: PAP. To insure full activity, the enzyme should be incubated in a... Read More

  • PAPL - Papain lyophilized, 100 mg

    Cat.-Nr: LS003119

    Supplied as a lyophilized powder prepared from a 2X crystalline suspension, Code: PAP. To insure full activity, the enzyme should be incubated in a... Read More

  • PAPL - Papain lyophilized, 25 mg

    Cat.-Nr: LS003118

    Supplied as a lyophilized powder prepared from a 2X crystalline suspension, Code: PAP. To insure full activity, the enzyme should be incubated in a... Read More

  • SI - Soybean Trypsin Inhibitor, purified

    Cat.-Nr: LS003570

    Chromatographically purified as described by Frattali, V., and Steiner, R.: Biochem., 7, 521 (1968). A dialyzed, lyophilized powder. Purity checked... Read More

  • SI - Soybean Trypsin Inhibitor, purified

    Cat.-Nr: LS003571

    Chromatographically purified as described by Frattali, V., and Steiner, R.: Biochem., 7, 521 (1968). A dialyzed, lyophilized powder. Purity checked... Read More

  • SIC - Soybean Trypsin Inhibitor

    Cat.-Nr: LS003587

    A partially purified lyophilized powder.

  • TRL - Trypsin 2x cryst.

    Cat.-Nr: LS003702

    Supplied as a chromatographically purified, diafiltered and lyophilized powder.

  • TRL - Trypsin 2x cryst.

    Cat.-Nr: LS003703

    Supplied as a chromatographically purified, diafiltered and lyophilized powder.

  • TRL - Trypsin 2x cryst.

    Cat.-Nr: LS003704

    Supplied as a chromatographically purified, diafiltered and lyophilized powder.

  • TRL3 - Trypsin 3x cryst.

    Cat.-Nr: LS003707

    Supplied as a chromatographically purified, diafiltered and lyophilized powder.

  • TRL3 - Trypsin 3x cryst.

    Cat.-Nr: LS003708

    Supplied as a chromatographically purified, diafiltered and lyophilized powder.

  • TRLS - Trypsin 3x cryst., filtered

    Cat.-Nr: LS003734

    Trypsin chromatographically purified, diafiltered (Code TRL3) filtered thorugh a 0.22 micron pore size membrane and lyophilized in sterile vials. ... Read More

  • TRLS - Trypsin 3x cryst., filtered

    Cat.-Nr: LS003736

    Trypsin chromatographically purified, diafiltered (Code TRL3) filtered thorugh a 0.22 micron pore size membrane and lyophilized in sterile vials.... Read More

  • TRLVMF - Trypsin, 2X, Irradiated

    Cat.-Nr: LS004452

    Chromatographically purified (Code: TRL), lyophilized, irradiated and tested for the absence of mycoplasma and extraneous virus according to 9... Read More

  • TRLVMF - Trypsin, 2X, Irradiated

    Cat.-Nr: LS004454

    Chromatographically purified (Code: TRL), lyophilized, irradiated and tested for the absence of mycoplasma and extraneous virus according to 9... Read More