Subculture & Reagents
Subculture & Reagents for optimal Cell Culture
Our Subculture & Reagents product line plays a vital role in optimizing growth conditions and providing essential propagation reagents for cell culture experiments.
Furthermore, they facilitate the seamless transition of cells from one vessel to another, ensuring their viability and functionality throughout the process.
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Specific Basal Media for a diverse array of Cell Types
A broad spectrum of Basal Media is available to support the growth and maintenance of various cell types.
From male and female reproductive cells to dermal cells such as keratinocytes and endothelial cells, specialized media formulations provide the optimal nutrient and growth factor environment for specific cell populations. These tailored media formulations promote cell health, proliferation, and functionality, ensuring reliable and reproducible results in cell culture experiments.
Single LifeFactors and LifeFactor Kits: Tailored Supplements for Basal Media
To further customize cell culture conditions, LifeFactors and LifeFactor Kits offer a convenient way to supplement the various Basal Media with specific additives and supplements. These kits contain carefully curated components designed to enhance cell growth, specific cellular functions, and partly also differentiation. Whether optimizing culture conditions for a particular cell type or investigating specific biological processes, LifeFactors and LifeFactor Kits provide researchers with the flexibility to tailor their cell culture protocols to their unique experimental needs.
Take advantage of our Growth Media Complete Kits, containing the optimized Basic Media and optimized LifeFactor Kit.
Trypsin-EDTA for Effective Cell Harvesting
Among the most essential subculture reagents is Trypsin-EDTA, a potent enzyme mixture used to detach adherent cells from the culture surface. By cleaving cell adhesion proteins,Ttrypsin-EDTA facilitates the gentle detachment of cells, ensuring high cell yields with minimal cellular stress. This reagent is indispensable for routine passaging of adherent cell lines and the establishment of new cultures.
Xeno-Free Trypsin-EDTA and more…
For researchers seeking to minimize the risk of introducing animal-derived components into their cell cultures, xeno-free trypsin-EDTA offers a viable solution. Xeno-free trypsin-EDTA provides the same robust performance as traditional formulations while meeting the stringent requirements of animal component-free cell culture systems. Xeno-Free Trypsin-EDTA contains human serum.
Also have a look at our Xeno-Free Human Primary Cells and Cell Culture Media.
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Bronchial Media LifeFactors Kit
Cat.-Nr: LS-1047
This kit contains the associated supplements and growth factors for use with Human Airway Epithelial Cells and BronchiaLife™ Basal Medium. With... Read More
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DermaLife K LifeFactors Kit
Cat.-Nr: LS-1030
Supplements and growth factors for use with Human Keratinocytes and DermaLife Basal Medium or DermaLife Calcium-Free Basal Medium. LifeFactors® are... Read More
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Dermalife M LifeFactors Kit
Cat.-Nr: LS-1041
Supplements and growth factors for use with Human Melanocytes -Neonatal and DermaLife Basal Medium. The LifeFactors® are packaged in a convenient... Read More
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DermaLife Ma LifeFactors Kit
Cat.-Nr: LS-1063
This kit includes the associated supplements and growth factors called “LifeFactors®” for use with Human Adult Melanocytes and DermaLife Basal... Read More
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Extract P™ Bovine Pituitary Extract, New Zealand Origin
Cat.-Nr: CM-0097
Extract P™ Bovine Pituitary Extract (BPE) Supplement Extract P™ is a protein-rich aqueous solution that has been extracted from bovine pituitary... Read More
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FibroLife S2 LifeFactors Kit
Cat.-Nr: LS-1038
Associated supplements and growth factors for use with FibroLife® Basal Medium for low-serum (2%) culture of Human Fibroblasts and Mesenchymal Stem... Read More
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FibroLife Serum-Free Fibroblast LifeFactors Kit
Cat.-Nr: LS-1010
Associated supplements and growth factors for use with Human Fibroblasts and FibroLife® Basal Medium. FibroLife Serum-Free LifeFactors... Read More
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FrostaLife Cryopreservation Solution
Cat.-Nr: LM-0015
FrostaLife™ is a solution optimized for the cryopreservation of mammalian cells by Lifeline® cell culture experts. Simply count your cells,... Read More
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MammaryLife LifeFactors Kit
Cat.-Nr: LS-1088
Growth factors and supplements for use with Human Mammary Epithelial Cells and Lifeline® MammaryLife™ Basal Medium. Packaged in a convenient... Read More
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OcuLife LifeFactors Kit
Cat.-Nr: LS-1057
Associated supplements and growth factors for use with Human Corneal Epithelial Cells and OcuLife™ Basal Medium. These growth factors and... Read More
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PBS w/o Ca / Mg
Cat.-Nr: CM-0001
Phosphate-buffered saline (PBS) maintains a constant pH, and its ion concentration and osmolarity matches that of human cells. PBS is used to remove... Read More
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ProstaLife LifeFactors Kit
Cat.-Nr: LS-1072
For use with human reproductive epithelial cells, including Prostate Epithelial Cells and Seminal Vesicle Epithelial Cells, and ProstaLife™ Basal... Read More
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RenaLife LifeFactors Kit
Cat.-Nr: LS-1048
For use with Human Renal Epithelial Cells and RenaLife™ Basal Medium. LifeFactors® (associated supplements and growth factors) are packaged in a... Read More
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ReproLife™ CX LifeFactors Kit
Cat.-Nr: LS-1116
ReproLife™ CX LifeFactors® are the associated supplements and growth factors for use with ReproLife ™ Basal Medium and Human Cervical Epithelial... Read More
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ReproLife™ LifeFactors Kit
Cat.-Nr: LS-1097
ReproLife™ LifeFactors® Kit contains associated supplements and growth factors called “LifeFactors®” for use with ReproLife™ Basal Medium... Read More
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StemLife MSC Lifefactors Kit
Cat.-Nr: LS-1060
Associated supplements and growth factors for use with StemLife MSC Basal Medium and Human Mesenchymal Stem Cells. StemLife MSC LifeFactors Kit... Read More
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StemLife MSC-BM LifeFactors Kit
Cat.-Nr: LS-1090
Associated supplements and growth factors for use with Bone Marrow Mesenchymal Stem Cells and StemLife MSC Basal Medium. LifeFactors® are packaged... Read More
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StemLife Sk LifeFactors Kit
Cat.-Nr: LS-1103
StemLife Sk LifeFactors® Kit includes associated supplements and growth factors for the growth of Human Skeletal Muscle Satellite Cells, with the... Read More
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TrypKit Xeno-Free
Cat.-Nr: LL-0043
TrypKit ™ Xeno-Free contains: CM-0001, PBS XF, 500 mL CM-0046 Trypsin EDTA XF, 100 mL CM-0047 Trypsin Neutralizing Solution XF, 100 mL
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TrypKit-100 ml
Cat.-Nr: LL-0013
Large TrypKit includes: Phosphate Buffered Saline (PBS) — 500 mL 0.05% Trypsin/0.02% EDTA (phenol red-free) — 100 mL Trypsin Neutralizing... Read More
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Trypsin 0.05% EDTA 0.02% (phenol red free)
Cat.-Nr: CM-0017
Trypsin acts as an enzyme to help dissociate cells from their culture vessel. EDTA is a calcium chelator that helps to prevent clumping of... Read More
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Trypsin Neutralizing Solution
Cat.-Nr: CM-0018
Trypsin is an enzyme that breaks down proteins to dissociate cells from the vessel in which they’re being cultured.
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Trypsin Neutralizing Solution Xeno-Free
Cat.-Nr: CM-0047
Trypsin neutralizing solution xeno-free acts as an enzyme to help dissociate cells from their culture vessel, and contains no non-human... Read More
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Trypsin/EDTA Xeno-Free
Cat.-Nr: CM-0046
Trypsin acts as an enzyme to help dissociate cells from their culture vessel. EDTA is a calcium chelator that helps to prevent clumping of... Read More
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Tryptic Soy Broth
Cat.-Nr: CM-0045
Tryptic Soy Broth (TSB) is a Culture broth used as a general medium to grow certain types of bacteria.
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UroLife™ LifeFactors Kit
Cat.-Nr: LS-1115
UroLife™ LifeFactors® Kit contains the associated supplements and growth factors for use with UroLife™ Basal Medium. UroLife™ is optimized... Read More
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VascuLife EnGS LifeFactors Kit
Cat.-Nr: LS-1019
Associated supplements and growth factors for use with Human Endothelial Cells and VascuLife® Basal Medium. LifeFactors® are packaged in a... Read More
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VascuLife EnGS-Mv LifeFactors Kit
Cat.-Nr: LS-1035
Supplements and growth factors for use with Human Microvascular Endothelial Cells and VascuLife® Basal Medium (endothelial cell culture... Read More
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VascuLife SMC LifeFactors Kit
Cat.-Nr: LS-1040
Associated supplements and growth factors for use with Human Smooth Muscle Cells and VascuLife® Basal Medium. LifeFactors® are packaged in a... Read More
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VascuLife VEGF LifeFactors Kit
Cat.-Nr: LS-1020
Associated supplements and growth factors for use with Human Endothelial Cells and VascuLife® Endothelial Basal Medium. LifeFactors® are packaged... Read More
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VascuLife VEGF-Mv LifeFactors Kit
Cat.-Nr: LS-1029
For use with Human Microvascular Endothelial Cells and VascuLife® Basal Medium. LifeFactors® are packaged in a convenient gas-impermeable pouch... Read More