Subculture & Reagents

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Subculture & Reagents

Our Subculture & Reagents product line plays a vital role in optimizing growth conditions and providing essential propagation reagents for cell culture experiments.

Furthermore, they facilitate the seamless transition of cells from one vessel to another, ensuring their viability and functionality throughout the process.

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A broad spectrum of Basal Media is available to support the growth and maintenance of various cell types.
From male and female reproductive cells to dermal cells such as keratinocytes and endothelial cells, specialized media formulations provide the optimal nutrient and growth factor environment for specific cell populations. These tailored media formulations promote cell health, proliferation, and functionality, ensuring reliable and reproducible results in cell culture experiments.

Single LifeFactors and LifeFactor Kits: Tailored Supplements for Basal Media

To further customize cell culture conditions, LifeFactors and LifeFactor Kits offer a convenient way to supplement the various Basal Media with specific additives and supplements. These kits contain carefully curated components designed to enhance cell growth, specific cellular functions, and partly also differentiation. Whether optimizing culture conditions for a particular cell type or investigating specific biological processes, LifeFactors and LifeFactor Kits provide researchers with the flexibility to tailor their cell culture protocols to their unique experimental needs.

Take advantage of our Growth Media Complete Kits, containing the optimized Basic Media and  optimized LifeFactor Kit.

Among the most essential subculture reagents is Trypsin-EDTA, a potent enzyme mixture used to detach adherent cells from the culture surface. By cleaving cell adhesion proteins,Ttrypsin-EDTA facilitates the gentle detachment of cells, ensuring high cell yields with minimal cellular stress. This reagent is indispensable for routine passaging of adherent cell lines and the establishment of new cultures.

For researchers seeking to minimize the risk of introducing animal-derived components into their cell cultures, xeno-free trypsin-EDTA offers a viable solution. Xeno-free trypsin-EDTA provides the same robust performance as traditional formulations while meeting the stringent requirements of animal component-free cell culture systems. Xeno-Free Trypsin-EDTA contains human serum.

Also have a look at our Xeno-Free Human Primary Cells and Cell Culture Media.

  • BronchiaLife LifeFactors Kit

    Cat.-Nr: LS-1047

    This kit contains the associated supplements and growth factors for use with Human Airway Epithelial Cells and BronchiaLife™ Basal Medium. With... Read More

  • DermaLife K LifeFactors Kit

    Cat.-Nr: LS-1030

    Supplements and growth factors for use with Human Keratinocytes and DermaLife Basal Medium or DermaLife Calcium-Free Basal Medium. LifeFactors® are... Read More

  • Dermalife M LifeFactors Kit

    Cat.-Nr: LS-1041

    Supplements and growth factors for use with Human Melanocytes -Neonatal and DermaLife Basal Medium. The LifeFactors® are packaged in a convenient... Read More

  • DermaLife Ma LifeFactors Kit

    Cat.-Nr: LS-1063

    This kit includes the associated supplements and growth factors called “LifeFactors®” for use with Human Adult Melanocytes and DermaLife Basal... Read More

  • Extract P™ Bovine Pituitary Extract, New Zealand Origin

    Cat.-Nr: CM-0097

    Extract P™ Bovine Pituitary Extract (BPE) Supplement Extract P™ is a protein-rich aqueous solution that has been extracted from bovine pituitary... Read More

  • FibroLife S2 LifeFactors Kit

    Cat.-Nr: LS-1038

    Associated supplements and growth factors for use with FibroLife® Basal Medium for low-serum (2%) culture of Human Fibroblasts and Mesenchymal Stem... Read More

  • FibroLife Serum-Free Fibroblast LifeFactors Kit

    Cat.-Nr: LS-1010

    Associated supplements and growth factors for use with Human Fibroblasts and FibroLife® Basal Medium.   FibroLife Serum-Free LifeFactors... Read More

  • FrostaLife Cryopreservation Solution

    Cat.-Nr: LM-0015

    FrostaLife™ is a solution optimized for the cryopreservation of mammalian cells by Lifeline® cell culture experts. Simply count your cells,... Read More

  • MammaryLife LifeFactors Kit

    Cat.-Nr: LS-1088

    Growth factors and supplements for use with Human Mammary Epithelial Cells and Lifeline® MammaryLife™ Basal Medium. Packaged in a convenient... Read More

  • OcuLife LifeFactors Kit

    Cat.-Nr: LS-1057

    Associated supplements and growth factors for use with Human Corneal Epithelial Cells and OcuLife™ Basal Medium. These growth factors and... Read More

  • PBS w/o Ca / Mg

    Cat.-Nr: CM-0001

    Phosphate-buffered saline (PBS) maintains a constant pH, and its ion concentration and osmolarity matches that of human cells. PBS is used to remove... Read More

  • ProstaLife LifeFactors Kit

    Cat.-Nr: LS-1072

    For use with human reproductive epithelial cells, including Prostate Epithelial Cells and Seminal Vesicle Epithelial Cells, and ProstaLife™ Basal... Read More

  • RenaLife LifeFactors Kit

    Cat.-Nr: LS-1048

    For use with Human Renal Epithelial Cells and RenaLife™ Basal Medium. LifeFactors® (associated supplements and growth factors) are packaged in a... Read More

  • ReproLife™ CX LifeFactors Kit

    Cat.-Nr: LS-1116

    ReproLife™ CX LifeFactors® are the associated supplements and growth factors for use with ReproLife ™ Basal Medium and Human Cervical Epithelial... Read More

  • ReproLife™ LifeFactors Kit

    Cat.-Nr: LS-1097

    ReproLife™ LifeFactors® Kit contains associated supplements and growth factors called “LifeFactors®” for use with ReproLife™ Basal Medium... Read More

  • StemLife MSC Lifefactors Kit

    Cat.-Nr: LS-1060

    Associated supplements and growth factors for use with StemLife MSC Basal Medium and Human Mesenchymal Stem Cells. StemLife MSC LifeFactors Kit... Read More

  • StemLife MSC-BM LifeFactors Kit

    Cat.-Nr: LS-1090

    Associated supplements and growth factors for use with Bone Marrow Mesenchymal Stem Cells and StemLife MSC Basal Medium. LifeFactors® are packaged... Read More

  • StemLife Sk LifeFactors Kit

    Cat.-Nr: LS-1103

    StemLife Sk LifeFactors® Kit includes associated supplements and growth factors for the growth of Human Skeletal Muscle Satellite Cells, with the... Read More

  • TrypKit Xeno-Free

    Cat.-Nr: LL-0043

    TrypKit ™ Xeno-Free contains: CM-0001, PBS XF, 500 mL CM-0046 Trypsin EDTA XF, 100 mL CM-0047 Trypsin Neutralizing Solution XF, 100 mL

  • TrypKit-100 ml

    TrypKit-100 ml

    Cat.-Nr: LL-0013

    Large TrypKit includes: Phosphate Buffered Saline (PBS) — 500 mL 0.05% Trypsin/0.02% EDTA (phenol red-free) — 100 mL Trypsin Neutralizing... Read More

  • Trypsin 0.05% EDTA 0.02% (phenol red free)

    Cat.-Nr: CM-0017

    Trypsin acts as an enzyme to help dissociate cells from their culture vessel. EDTA is a calcium chelator that helps to prevent clumping of... Read More

  • Trypsin Neutralizing Solution

    Cat.-Nr: CM-0018

    Trypsin is an enzyme that breaks down proteins to dissociate cells from the vessel in which they’re being cultured.

  • Trypsin Neutralizing Solution Xeno-Free

    Cat.-Nr: CM-0047

    Trypsin neutralizing solution xeno-free acts as an enzyme to help dissociate cells from their culture vessel, and contains no non-human... Read More

  • Trypsin/EDTA Xeno-Free

    Cat.-Nr: CM-0046

    Trypsin acts as an enzyme to help dissociate cells from their culture vessel. EDTA is a calcium chelator that helps to prevent clumping of... Read More

  • Tryptic Soy Broth

    Cat.-Nr: CM-0045

    Tryptic Soy Broth (TSB) is a Culture broth used as a general medium to grow certain types of bacteria.

  • UroLife™ LifeFactors Kit

    Cat.-Nr: LS-1115

    UroLife™ LifeFactors® Kit contains the associated supplements and growth factors for use with UroLife™ Basal Medium. UroLife™ is optimized... Read More

  • VascuLife EnGS LifeFactors Kit

    Cat.-Nr: LS-1019

    Associated supplements and growth factors for use with Human Endothelial Cells and VascuLife® Basal Medium. LifeFactors® are packaged in a... Read More

  • VascuLife EnGS-Mv LifeFactors Kit

    Cat.-Nr: LS-1035

    Supplements and growth factors for use with Human Microvascular Endothelial Cells and VascuLife® Basal Medium (endothelial cell culture... Read More

  • VascuLife SMC LifeFactors Kit

    Cat.-Nr: LS-1040

    Associated supplements and growth factors for use with Human Smooth Muscle Cells and VascuLife® Basal Medium. LifeFactors® are packaged in a... Read More

  • VascuLife VEGF LifeFactors Kit

    Cat.-Nr: LS-1020

    Associated supplements and growth factors for use with Human Endothelial Cells and VascuLife® Endothelial Basal Medium. LifeFactors® are packaged... Read More

  • VascuLife VEGF-Mv LifeFactors Kit

    Cat.-Nr: LS-1029

    For use with Human Microvascular Endothelial Cells and VascuLife® Basal Medium. LifeFactors® are packaged in a convenient gas-impermeable pouch... Read More