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Home |Products|Other Products|Staining Kits|Cytus™ Green / Red Cell Viability Kit (Calcein AM & Propidium Iodide)

Cytus™ Green / Red Cell Viability Kit (Calcein AM & Propidium Iodide)

Cat.-Nr.: 5471-1KIT

Cytus™ Green / Red Cell Viability Kit (Calcein AM + Propidium Iodide)
Viable human fibroblasts stained with Cytus™ Green.Nonviable human fibroblasts stained with Cytus™ Red.

Description

Dual-color cell viability assay combining Calcein AM and Propidium Iodide for rapid, high-contrast viability assessment. Cytus™ Green labels metabolically active cells, while Cytus™ Red stains dead cells, enabling accurate imaging, cytotoxicity studies, and quantitative analysis.

The Cytus™ Green / Red Cell Viability Kit is a dual-color fluorescence assay designed for rapid and reliable assessment of live and dead cells. It combines Cytus™ Green Calcein AM for viable cells with Cytus™ Red Propidium Iodide for non-viable cells, enabling simultaneous discrimination within mixed populations.

Cytus™ Green is a membrane-permeable, non-fluorescent compound that is enzymatically converted into fluorescent calcein in metabolically active cells. This conversion requires intact membranes and intracellular esterase activity, resulting in strong green fluorescence proportional to viable cell number.

Cytus™ Red selectively stains dead or membrane-compromised cells by intercalating into nucleic acids, producing bright red fluorescence upon binding. Together, these dyes provide a robust and widely validated method for viability assessment across a range of experimental systems.

Parameter, Testing, and Method Cytus™ Green / Red #5471-1KIT
Live Cell Dye Green (Cytus™ Green Calcein AM)
Dead Cell Dye Red (Cytus™ Red Propidium Iodide)
Stock Concentration Green/Red: 1 mM in DMSO
Stock Volume Green/Red: 100 µL
Appearance Green: yellow hue, Red: red hue; clear liquid
Sterility Non-Sterile
Assay Type Live dead cell viability
Cell Compatibility Live and dead cells
Working Concentration Green: 2 µM; Red: 2 µM
Excitation/Emission (Green) 501 / 521 nm
Excitation/Emission (Red) 537 / 617 nm
Storage Temperature -20°C
Shelf Life Minimum of 6 months from date of receipt

  • SUPPLIER:

    Advanced BioMatrix

  • STATUS:

    In Stock

  • SIZE:

    2x100 µl, 500 tests

  • Overview
  • Related Files
  • References

Overview

Dual-color cell viability assay combining Calcein AM and Propidium Iodide for rapid, high-contrast viability assessment.
  • Features: Cell Imaging Reagents, solution
  • Features:Dual-Color Fluorescence Staining Solution

Related Files

Datasheet

References

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Link to: hiEx™ Research iPSC Exosome Services Link to: hiEx™ Research iPSC Exosome Services hiEx™ Research iPSC Exosome ServicesMicroscopic Image of an in vitro wound healing assay using human dermal fibroblasts at 24 hours showing progressive wound closure with hiEX™ Research iPSC Exosomes treatment, cells imaged at 50x magnification. Link to: Cytus™ Green Calcein AM, 100µl Link to: Cytus™ Green Calcein AM, 100µl Cytus™ Green , a calcein AM–based stain for viable cellsCytus™ Green Calcein AM, 100µl
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