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Home |Products|Coagulation Proteins|Human Glu-Plasminogen

Human Glu-Plasminogen

Cat.-Nr.: HCPG-0130

Description

Plasminogen is a single chain glycoprotein zymogen which is synthesized in the liver and circulates in plasma at a concentration of approximately 2.4 µM (1,2). The plasminogen molecule contains 790 amino acids, 24 disulfide bridges, no free sulfhydryls and 5 regions of internal sequence homology, known as kringles, between Lys77 and Arg560. These five triple-looped, three disulfide bridged, kringle regions are homologous to the kringle domains in t-PA, u-PA and prothrombin. Plasminogen contains one high affinity (Kd = 9×10-6M) and four low affinity (Kd = 5×10-3M) lysine binding sites. The high affinity binding site resides within the first kringle region of plasminogen. The interaction of plasminogen with fibrin and 2-antiplasmin is mediated by these lysine binding sites. Native glu-plasminogen (Mr=88,000) is readily converted to Lys-77-plasminogen (Mr = 83,000) by plasmin hydrolysis of the Lys76-Lys77 peptide bond. Elastase catalyzed cleavage of the Val441-Val442 peptide bond of glu-plasminogen yields a functionally active zymogen termed Val-442 plasminogen or mini-plasminogen. The conversion of plasminogen to plasmin occurs by a variety of mechanisms, but all result in hydrolysis of the Arg560-Val561 peptide bond of plasminogen, yielding two chains which remain covalently associated by a disulfide bond. Native glu-plasminogen is prepared from fresh frozen human plasma by a modification of the procedure of Castellino (3), utilizing gel filtration and affinity chromatography. The two carbohydrate variants of glu-plasminogen (CHOI and CHOII) are isolated by gradient elution from lysine-Sepharose using the lysine analog, -aminocaproic acid (3). The plasminogen is supplied in 50% (vol/vol) glycerol/H2O for storage at -20°C. Purity is determined by SDS-PAGE analysis.

  • SUPPLIER:

    Haematologic TechnologiesProlytix

  • STATUS:

    In Stock

  • SIZE:

    1 mg

  • Overview

Overview

  • Species: Human
  • Additional Attributes: Zymogenes
  • Formulation:50% (vol/vol) glycerol/H2O
  • Purity:>95%
  • Localization:Plasma
  • Concentration of plasma:2.4 µM
  • Mode of action:Zymogen; precursor to the serine protease plasmin
  • Molecular Weight:88000
  • Extinction Coeff:17
  • Isoelectric point:06. Feb
  • Structure:single chain, 24 intra chain disulfide bridges, 5 kringle regions.
  • Percent carbohydrate:~2%

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Human Glu-Plasminogen (affinity form I) Human gamma-thrombin
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