- Species: Human
- Additional Attributes: iPSC-derived cells
- Specific Attributes: frozen
Applied StemCell’s Neurons are cryo-preserved, pre-differentiated mixed population neuronal precursors derived from a footprint-free, karyotype normal human iPSC line (male cord-blood-derived). They yield high purity, functional neurons within 8 days when using Applied StemCell’s well-optimized maturation medium and supplements. The derived-neurons have also been tested for neuronal activity using functional and screening assays. The neurons can be seeded on various culture vessel formats including 96-well plates on either glass or plastic surfaces and cultured as adherent cells. Shortly after seeding, the cells proliferate slightly for up to 3 days and show extensive neurite outgrowth and proper neuronal morphology. In general, on Day 8 post-seeding, the cell population will contain >90% neurons and < 5% Glial Fibrillary Acidic Protein (GFAP) positive astrocytes.