DOPA Maturation Media is serum-free media produced using Applied StemCell’s proprietary formulations to allow researchers to enable robust maturation and maintenance of dopaminergic precursor cells into functional dopaminergic neurons (DOPA). Applied StemCell’s induction and maturation media have been tested and optimized using NSC and neurons derived from Applied StemCell’s control iPSC line, ASE-9109 and ASE-9110. The DOPA neuron differentiation process is divided into two stages: In the first stage, the DOPA Induction Medium (ASE-9323DI) is used to differentiate NSC into DOPA precursors. In the second stage, the DOPA Maturation Medium is used to further differentiate the precursors into high purity, mature, functional DOPA neurons and to maintain these neurons in long-term culture (up to 40 days) (See Figure 1). DOPA Induction Medium can be purchased separately (ASE-9323DI) or as part of the Dopaminergic Neurons Starter Kit (ASE-9323K/ ASE-9323KF).