DOPA Induction Media is serum-free media produced using Applied StemCell’s proprietary formulations to allow researchers to differentiate neural stem cells (NSC) into functional dopaminergic neurons (DOPA). The DOPA neuron differentiation process is divided into two stages. In the first stage, the DOPA Induction Medium is used to differentiate NSC into DOPA precursors. In the second stage, the DOPA Maturation Medium is used to further differentiate the precursors into mature, functional DOPA neurons and to maintain these neurons in long-term culture (up to 40 days) (See Figure 1). DOPA Maturation Medium can be purchased separately (ASE-9323DM) or as part of the Dopaminergic Neurons Starter Kit (ASE-9323/ ASE-9323F).