Human Astrocyte Precursor Kit contains cryo-preserved, pre-differentiated astrocyte precursors derived from a footprint-free, karyotype normal human iPSC line (ASE-9109). It is designed for customers to generate mature astrocytes using ASC’s optimized maturation medium (ASE-9322MDM) and supplements. Mature astrocytes can be obtained within 17 days. Shortly after seeding and recovery, the cells proliferate and need to be passaged a minimum of two times. In general, on day 17 post-seeding, the cell population will contain =80% Glial Fibrillary Acidic Protein (GFAP) positive astrocytes and = 15% Tuj-1 positive neurons (neuronal class III ß-tubulin).